Journal: Sensors (Basel, Switzerland)

Article Title: Human Thrombin Detection Through a Sandwich Aptamer Microarray: Interaction Analysis in Solution and in Solid Phase

doi: 10.3390/s111009426

Figure Lengend Snippet: Electrophoretic Mobility Shift Assay (EMSA) of unmodified TBA1 and thrombin in presence (a) and in absence of Potassium (b) . TBA1 was incubated with the protein under the conditions described. We have indicated in the figure the respective TBA1 and thrombin concentrations. A control reaction without thrombin was performed in all experiments. KCl was added to the polyacrilamide gel and to the running buffer. Binding reactions were applied on a 12% non-denaturing PAA gel containing 1X TBE buffer and KCl 10 mM. The mobility of free and complexed aptamers, stained by SybrGreen II®, was detected using the Geliance 600 Imaging System.

Article Snippet: Aptamers on the gels were stained with the fluorescent DNA binding dye SybrGreen II ® (Invitrogen) that preferentially binds to single-stranded DNA and emits fluorescence (λ exc = 488 nm; λ em = 522 nm) when in complex with DNA.

Techniques: Electrophoretic Mobility Shift Assay, Incubation, Control, Binding Assay, Staining, Imaging

Journal: Sensors (Basel, Switzerland)

Article Title: Human Thrombin Detection Through a Sandwich Aptamer Microarray: Interaction Analysis in Solution and in Solid Phase

doi: 10.3390/s111009426

Figure Lengend Snippet: Electrophoretic Mobility Shift Assay (EMSA) of TBA1-NH 2 with thrombin (a) and of TBA1(12T)NH 2 with thrombin (b) . TBA1-NH 2 and TBA(12T)NH 2 were separately incubated with the protein under the conditions described. We have indicated in the figure the respective aptamer and thrombin concentrations. A control reaction without thrombin was performed in all experiments. Binding reactions were applied on a 12% non-denaturing PAA gel containing 1X TBE buffer and KCl 10mM. The mobility of free and complexed aptamers, stained by SybrGreen II®, was detected using the Geliance 600 Imaging System.

Article Snippet: Aptamers on the gels were stained with the fluorescent DNA binding dye SybrGreen II ® (Invitrogen) that preferentially binds to single-stranded DNA and emits fluorescence (λ exc = 488 nm; λ em = 522 nm) when in complex with DNA.

Techniques: Electrophoretic Mobility Shift Assay, Incubation, Control, Binding Assay, Staining, Imaging

Journal: Sensors (Basel, Switzerland)

Article Title: Human Thrombin Detection Through a Sandwich Aptamer Microarray: Interaction Analysis in Solution and in Solid Phase

doi: 10.3390/s111009426

Figure Lengend Snippet: Electrophoretic Mobility Shift Assay (EMSA) of unmodified TBA2 with thrombin (a) and TBA2Cy5 with thrombin (b) . Each aptamer was incubated with the protein under the conditions described. We have indicated in the figure the respective aptamer and thrombin concentrations. A control reaction without thrombin was performed in all experiments. Binding reactions were applied on a 12% non-denaturing PAA gel containing 1X TBE buffer and KCl 10 mM. The mobility of free and complexed aptamers, stained by SybrGreen II ® (a,b) was detected using the Geliance 600 Imaging System.

Article Snippet: Aptamers on the gels were stained with the fluorescent DNA binding dye SybrGreen II ® (Invitrogen) that preferentially binds to single-stranded DNA and emits fluorescence (λ exc = 488 nm; λ em = 522 nm) when in complex with DNA.

Techniques: Electrophoretic Mobility Shift Assay, Incubation, Control, Binding Assay, Staining, Imaging